Glycolysis without pyruvate kinase in Clostridium thermocellum
نویسندگان
چکیده
منابع مشابه
Atypical glycolysis in Clostridium thermocellum.
Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate (PPi)-linked activity. Phosphoglycerate kinase used both GDP and ADP as phosphor...
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In Clostridium thermocellum, a thermophilic anaerobic bacterium able to rapidly ferment cellulose to ethanol, pyruvate kinase (EC 2.7.1.40) is absent based on both the genome sequence and enzymatic assays. Instead, a new pathway converting phosphoenolpyruvate to pyruvate via a three-step pathway involving phosphoenolpyruvate carboxykinase, NADH-linked malate dehydrogenase, and NADP-dependent ma...
متن کاملEnhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase
BACKGROUND Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. RESULT Here we examined the thermostability of eight PDCs. The purified bacterial enzymes retained 20% of activity after incubation for 30 min at 55 °C. Expression of these PDC genes, except the one from Zymomonas ...
متن کاملElectrotransformation of Clostridium thermocellum.
Electrotransformation of several strains of Clostridium thermocellum was achieved using plasmid pIKm1 with selection based on resistance to erythromycin and lincomycin. A custom-built pulse generator was used to apply a square 10-ms pulse to an electrotransformation cuvette consisting of a modified centrifuge tube. Transformation was verified by recovery of the shuttle plasmid pIKm1 from presum...
متن کاملPhosphorylation of pyruvate by the pyruvate kinase reaction and reversal of glycolysis in a reconstructed system.
Lardy and Ziegler (1) obtained synthesis of phosphoenol pyruvate from pyruvate and adenosine triphosphate by reversal of the pyruvate kinase reaction. A possible mechanism was thus demonstrated for the initial step in the synthesis of glycogen from pyruvate. The amounts of phosphoenol pyruvate synthesized were, however, small, and it is rather widely held that reversal of the pyruvate kinase re...
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ژورنال
عنوان ژورنال: Metabolic Engineering
سال: 2017
ISSN: 1096-7176
DOI: 10.1016/j.ymben.2016.11.011